The Instructions for Use (IFU) detail the procedure to properly wet out the capsules. This will involve priming buffer through the inlet of the capsule upside down, so air is expunged through the outlet facing up. If air is trapped in the membrane, the binding capacity could be compromised.
The operator is advised not to introduce air into the capsule once the capsule is wetted. If operating capsules on a liquid chromatography (LC) system, then all buffer and sample lines must be properly primed to ensure no air bubbles are loaded onto the membrane capsule. In such cases where air can be trapped during the loading step, we recommend installing a bubble trap inline (for example a three-way valve to release any air) prior to loading into the membrane.
Mustang™ membranes (Q, S, and E) are compatible with 1 N NaOH. However, the housings used for the disposable formats are stable for one cycle only (30 min contact time at 20˚C).
The reusable Mustang™ XT capsules (Pall™ Life Sciences products) are all polypropylene and are compatible with several cycles of 1 N NaOH. The number of cycles is sample specific and must be determined empirically.
10 membrane volumes/min (MV/min) is typical. However, for large molecules such as viral vectors, 5 MV/min or less is typical to prevent a large drop in pressure.
We recommend a 0.1 N NaOH/1 N NaCl solution for storage. For additional details, see the Membrane Chromatography Validation Guides
Mustang™ Q membrane chromatography – 0.8 µm modified hydrophilic polyethersulfone (PES)
Mustang™ S membrane chromatography – 0.65 µm modified hydrophilic PES
Mustang™ E membrane chromatography – 0.2 µm modified hydrophilic PES
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