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Critical for Analytical: Sample Preparation

Reliable Results with Superior Protection

Whether pursuing analytical goals in life science, pharmaceutical methods, research and development, quality control, or environmental applications, Pall’s sample preparation filtration devices ensure reliable and reproducible results.

 

Protecting your instrumentation

 

Filtering analytical samples and mobile phase solutions using the correct filtration device is an economical way to reduce instrument downtime, maintenance and associated costs.

 

Sample filtration prolongs injector life, prevents the blocking of capillaries and protects detector cells from particulate contamination.

 

Mobile phase filtration will both degas and remove unwanted particulate. Contamination can cause the plugging of the sinker and column frits, may damage pump valves and block capillaries.

Filtration of both samples and mobile phases will also prevent microbial growth that can cause increased baseline noise.

 

Protecting your column

 

Proper analytical chromatography column selection and integrity is crucial for efficient compound separation and identification. The packing of high-performance columns is composed of small particles of narrow size distribution.

 

Injection of contaminated particulate-laden samples into columns will block the flow path between the packing and impact column integrity, causing loss of resolution, poor peak shape, split peaks and high back pressure. Therefore, making analytical results difficult to interpret and shortening column life.

 

Sample filtration using a membrane of the correct pore size will prevent particulate contamination from blocking. For example, if a column packing is 3 μm in diameter then, using simple mathematics, it is possible to calculate that the flow path is 0.43 μm, therefore a 0.2 μm filter would prevent particulate from plugging the column.

 

Protecting your results

 

Filtration helps ensure the integrity of your instrument and columns, offering you more reliable and reproducible results. However, when filtering a sample, solvent or mobile phase solution it’s important that the filtration device does not interfere with your analytes, either by adding contaminants or by binding to analytes.

 

With over 70 years of membrane development expertise, Pall’s proprietary filtration technology delivers excellent quality and reliability that you can count on. Our accurate pore size ratings offer better chromatography, and instrument and column protection. The Acrodisc One™ syringe filters with wwPTFE can extend column life by up to 52 times.

 

 

Effects of filters on HPLC column life following injections of unfiltered and filtered 0.05% latex sphere suspensions. With unfiltered samples, the column failed due to plugging after 19 injections. Samples passed through competitor filters plugged the columns after 500 injections. No increase in backpressure was observed after 1000 injections of samples filtered with Pall Acrodisc One syringe filters.

 

 

Our HPLC, LCMS and IC certified sample prep filtration devices have been designed, manufactured and tested to ensure low levels of extractables.

 

HPLC Certification

Pall HPLC certification ensures that analytical results will not be compromised by extractable filter materials. Our membranes have been tested for compatibility with the common HPLC solvents water, methanol, and acetonitrile, using established HPLC procedures. In addition, to verify low levels of UV-detectable extractables, samples of the entire HPLC Acrodisc syringe filter line are evaluated prior to release.

 

LCMS Certification

Pall certifies that all lots of water wettable polytetrafluoroethylene (WWPTFE) membrane used in the Acrodisc MS syringe filters have been tested according to established LCMS techniques. Each box of product comes with a certificate containing Total Ion Current (TIC) chromatograms that show all detected peaks relative to an Internal Standard. Results for integrity, burst, and flow rate tests are also included on the certificate.

 

IC Certification

Pall Laboratory confirms that Ion Chromatography (IC) Acrodisc syringe filters have been tested using a highly sensitive IC protocol to monitor inorganic extractables. For IC applications, our IC Acrodisc polyethersulfone (PES) syringe filters are certified for low levels of inorganic extractables. Actual background levels of filter extractables are typically less than 20 ppb for chloride, 6 ppb for nitrate, 1 ppb for phosphate, and 10 ppb for sulfate.

 

Chemical compatibility is a critical consideration when selecting the best sample prep syringe filter or mobile phase disc filter for your application. Pall provide a range of analytical filter membranes, this includes our wwPTFE membrane which is compatible with the majority of common solvent and sample types.

 

Pall’s superior media separation technology ensures:

 

  • Accurate pore size ratings for better chromatography, and instrument and column protection
  • Uniform membrane materials (lot-to-lot) for consistent analytical results
  • Materials of construction selected specifically for analytical analysis with lower extractables and lower API adsorption
Media Selection Proteinaceous General Aqueous Non-Aggressive Organic Aggressive Organic

wwPTFE (water wettable hydrophillic polytetrafluoroethene) 

wwPTFE is a hydrophilic membrane compatible with the majority of mobile phase solutions and solvents including, aqueous, acidic, basic, non aggressive organic, and aggressive organic solutions. wwPTFE membrane offers low protein binding and low levels of UV-absorbing extractables.

🟢 🟢 🟢 🟢

PTFE Membrane

Exceptional chemical and temperature compatibility make this membrane ideal for harsh chemicals that destroy other membrane materials.

🟥 🟥 🟢 🟢

Nylon Membrane

This versatile filter for both aqueous and solvent-based sample filtration exhibits excellent chemical compatibility with esters, bases, and alcohols Not recommended for acids 1 N or halogenated solvents.

🟡** 🟢 🟡 🟥

PVDF Membrane

Compatible with many organic and non-organic solvents making it ideal for a wide range of applications.

Not recommended for acetone, DMF, DMSO, or bases >6 N.

🟢 🟢 🟡 🟥

Glass Fiber Media

Maximize throughput for extremely particulate-laden samples.

🟢 🟢 🟡 🟥

Supor® (PES) Membrane for lon Chromatography (IC) 

Optimized to provide the most consistent results when analyzing ionic species, even for the most sensitive analyses. Certified for low levels of ionic extractables.

🟢 🟢 🟡 🟥

Hydrophilic Membranes for General Aqueous Samples

Widely used in dissolution testing, choose from a variety of pore sizes and membrane types for prefiltration and clarification including Supor, and Versapor membranes.

🟢 🟢 🟡 🟥
🟢 Recommended 🟡 Suitable 🟥 Not Recommended ** Dependent on protein type and concentration

Pall offer a variety of products made from the same materials of construction, allowing for single or multiple sample processing for your analytical techniques. We bring together membranes with superior performance, outstanding housing materials, and devices designed to maximize processing accuracy and speed.

 

Our analytical sample preparation devices are available with optimized effective filtration areas that maximize the filtration throughput of your sample while minimizing sample hold-up volume.

 

Product AcroPrep™ Advance 350 μL Filter Plate Nanosep® MF Device AcroPrep Advance 1 mL Filter Plate AcroPrep Advance 2 mL Filter Plate
Sample Volume ≤ 300 μL ≤ 500 μL ≤ 900 μL ≤ 1.9 mL
Membrane Choice
wwPTFE 🟢 🟢 🟢 🟢
PTFE 🟢   🟢 🟢

 

 

Product 4 mm Acrodisc Syringe Filter 13 mm Acrodisc Syringe Filter 25 mm Acrodisc Syringe Filter 25 mm Acrodisc PSF Syringe Filter
       
Sample Volume ≤ 2 mL
≤ 10 mL
≤ 100 mL
≤ 125 mL
Membrane Choice
wwPTFE   🟢 🟢 🟢
PTFE 🟢 🟢 🟢 🟢
Nylon   🟢 🟢 🟢
PVDF   🟢 🟢 🟢
PES   🟢 🟢 🟢
Vesapor   🟢   🟢
Supor   🟢 🟢 🟢
Glass Fiber       🟢