Following identification of clones expressing the protein of interest, clonal amplification, cell harvest, media collection and sampling is required. Streamlining these processes through efficient sample preparation and purification of the biomolecules for analysis can save significant time and resources. Comprehensive characterization of the expressed target protein in terms of yield, purity, stability, structure, and function is essential to select suitable clones for further optimization and scale-up. To aid in this determination several
techniques can be used, including next generation sequencing, immunohistochemistry, and mass spectroscopy.
Our high-performance depth filter media efficiently removes cells and clarifies cell lysates with low hold-up volumes, increasing product recovery.
Contaminants in biotherapeutics are of great concern. Endotoxin, a bacterial toxic liposaccharide (LPS), can be introduced from plasmid preparations and bacterial protein expression. Endotoxin must be removed to avoid toxicity and interference with downstream cell-based assays. While intact bacteria can be captured using a 0.2 µm filter, LPS are much smaller and can be more challenging to capture. An effective method of removal of the negatively charged LPS is to use a substrate with a positive charge. Pall offer Acrodisc® Units with Mustang® E Membrane for effective removal of endotoxin.
Our simple-to-use, innovative nucleic acid binding centrifugal devices, incorporate double layer glass fiber membranes, providing high yields and the flexibility to purify genomic DNA and mRNA in a single device to support multiple downstream applications. Pall nucleic acid purification solutions save time by removing unnecessary steps. The automation-friendly and high throughput 96-well format eliminates the need for sequential sample preparations further increasing the processing speed of workflows.
Pall offers high-sensitivity protein binding membranes designed for protein detection analysis using techniques such as western blotting. Low background noise ensures clarity and sensitivity of results. Immobilized proteins can be used directly for sequencing, providing flexibility in the workflow.