Growing and culturing of cells are the most fundamental steps in clone screening. The health of the cells is critical to optimizing the production of the protein of interest. Both adherent and suspension cells must be grown in an aseptic environment to ensure no contaminant is introduced that could render the batch unusable or reduce the integrity of subsequent analysis. In addition to an aseptic environment, growing media and nutrients supplemented to the cells must be sterile or else risk contamination.
Pall Laboratory offers a complete range of 0.2 µm sterile grade filtration solutions for cell culture applications from small feed volumes of a few milliliters, to over 100 liters. Our proprietary dual layer membranes enable fast and efficient filtration of nutrient rich media, feed, and supplements. Pall filtering media are low protein binding, ensuring valuable nutrients are not lost during sterile filtration. Sterile syringe filters allow small samples to be taken for assessment when monitoring culture growth without risk of contamination.
Mycoplasmas are a common contaminant of cell cultures but are not readily discernible by routine laboratory methods. Using our 0.1 µm Supor® mycoplasma reduction membrane is the best assurance to limit potential mycoplasma from being introduced into cell cultures by serum or serum-containing media. Pall Laboratory has filter products suitable to most workflow needs throughout the cell passaging and bioreactor culture process.