In addition to identifying clone candidates that stably produce the biotherapeutic protein, the purity, titer and biological activity of the biomolecule is analyzed as part of an iterative process to optimize production. Functional analysis includes protein transcription, expression, structure and cellular interaction studies through techniques such as polyacrylamide gel electrophoresis (SDS-PAGE), Liquid Chromatography–Mass Spectrometry (LC-MS) and High-Performance Liquid Chromatography (HPLC).
Pall Laboratory offers innovative nucleic acid binding centrifugal devices which simplify protein sample preparation, gDNA and mRNA purification prior to LC-MS or HPLC analysis. The Nanosep® Centrifugal Filter incorporates a high-binding innovative double layer glass fiber membrane. High-quality and quantity yields with even recovery of fragments as small as 50 bp up to 10, 000 bp, support multiple downstream applications, while saving time and resource by removing the need to process additional samples.
High-sensitivity, low background noise membranes designed for protein analysis applications, such as western blotting, provide clarity of results. Immobilized proteins to be used directly for sequencing increasing flexibility in your workflow. Products are optimized to reduce extractables and pre-sterilization by gamma irradiation eliminates potential cytotoxic residues.