The goal of the purification step in clone optimization is to enhance recovery of the target biomolecule for detailed functional analysis. Contaminants such as whole cell proteins, DNA, or unwanted mAb variants are removed through a series of chromatographic steps. The process can be quickened, and cost reduced, by concentrating the solution prior to purification.

 

To ensure the target proteins are in the desired solution for

purification or analysis, diafiltration, a process of simultaneous buffer exchange and concentration can be performed before or after the chromatography step.

 

Ultrafiltration via Pall Laboratory Tangential Flow Filtration (TFF) systems provide efficient processing, concentration and diafiltration (desalting or buffer exchange) on the same system with minimal user intervention. Optimized flow path design coupled with low protein-binding membranes minimize non-specific binding for high product recovery. Our scalable range of TFF capsules delivers predictable performance saving time when scaling up the process. Concentration and buffer exchange can be done simultaneously in a process called ‘continuous-diafiltration’ which can save hours of processing time.